ThisaplicationisalsorelatedtoInternationalPublica generationofthefunctionalprotein,eachsteprepresentsantionNo.PCT/US2012/58519,filedOct.3,2012,entitled35oportunityforeroranddamagetothecel.Further,itis ModifiedNucleosides,Nucleotides,andNucleicAcids,and knowntobedificulttoobtainDNAexpresionincelsasUsesThereofandInternationalPublicationNo.PCT/ DNAfrequentlyentersacelbutisnotexpresedornot US2012/69610,filedDec.14,2012,entitledModified expresedatreasonableratesorconcentrations.ThiscanbeNucleoside,Nucleotide,andNucleicAcidCompositions. aparticularproblemwhenDNAisintroducedintoprimary Theinstantaplicationisalsorelatedtoco-pending40 celsormodifiedcellines.aplications,eachfiledconcurentlyherewithonMar.9, Intheearly190’sBlom andcoleaguessucesfuly 2013andhavingInternationalPublicationNo.WO/2013/1516,entitledModifiedPolynucleotidesfortheProduc rescuedvasopresin-deficientratsbyinjectinginvitro tionofBiologicsandProteinsAsociatedwithHuman transcribedvasopresinmRNAintothehypothalamus(Scihttps://www.modernatx.com/sites/default/files/US10703789.pdf Disease;InternationalPublicationNo.WO/2013/151667, ence25:96-98;1992).However,thelowlevelsof 45 entitledModifiedPolynucleotides;InternationalPublication translationandtheimunogenicityofthemoleculesham No.WO/2013/151663,entitledModified Polynucleotides for the Production of Membrane Proteins; International Publication No. PCT/US2013/151669, […]